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Schematic representation of vector/target recombination events using the...
Schematic representation of vector/target recombination events using the positive-negative selection approach. (A) Random vector insertion. (B) Gene targeting via homologous recombination (HR). (C)...
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Schematic representation of gene targeting in ESC.
Schematic representation of gene targeting in ESC.
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Microinjection of DNA solution into one of the pronuclei of a mouse zygote.
Microinjection of DNA solution into one of the pronuclei of a mouse zygote.
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Genetic and Phenotypic Characteristics of Various Human TNF Transgenic Mouse...
Genetic and Phenotypic Characteristics of Various Human TNF Transgenic Mouse Lines Generated in Our Laboratory
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DNA substrates recommended for binding and annealing assays
DNA substrates recommended for binding and annealing assays
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Kinetics of Rad52-mediated strand annealing: (a) Representative raw data...
Kinetics of Rad52-mediated strand annealing: (a) Representative raw data from a typical annealing experiment. Fluorescence intensity of Cy3 (black) and Cy5 (gray) are recorded in kinetics mode every...
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Binding of Rad52 protein to oligonucleotides of different lengths. (a)...
Binding of Rad52 protein to oligonucleotides of different lengths. (a) Binding titrations with human Rad52 and 1 nM ssDNA of various lengths were performed and the FRET efficiency (E FRET) was...
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Rad52 binding to the RPA–ssDNA complex: (a) Raw data from a representative...
Rad52 binding to the RPA–ssDNA complex: (a) Raw data from a representative experiment. Fluorescence intensity of Cy3 (black) and Cy5 (gray) are recorded in kinetic mode every 1 s. Arrows above the...
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RPA–ssDNA binding experiment: (a) Raw data from a representative experiment....
RPA–ssDNA binding experiment: (a) Raw data from a representative experiment. Fluorescence intensity of Cy3 (black) and Cy5 (gray) are recorded in kinetic mode every 1 s. Arrows above the graph...
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The Rad52 oligomer forms a ring structure with ssDNA binding site spanning...
The Rad52 oligomer forms a ring structure with ssDNA binding site spanning along the perimeter: structure of the undecameric ring of the DNA binding domain of human Rad52 protein (coordinates were...
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Outcomes of the repair of a double-strand break (DSB) introduced by I-SceI....
Outcomes of the repair of a double-strand break (DSB) introduced by I-SceI. (A) The integrated pim1-Q vector. Open arrows indicate the positions of PCR primers that are used to distinguish between the...
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Schematic representations of the (A) pim1-Q and (B) Rb-O targeting vectors....
Schematic representations of the (A) pim1-Q and (B) Rb-O targeting vectors. (A) Gene targeting strategy at the pim1 locus. Expression of the promotorless hygromycin resistance relies on the endogenous...
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The scalloped wing phenotype indicates deletion into sequences flanking P{w...
The scalloped wing phenotype indicates deletion into sequences flanking P{w a } during repair. (a) Wild-type wing structure in a female fly. (b) A mild scalloped-wing phenotype resulting from a small...
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Cross schemes for the P{w a } assay when testing the role of the DmBlm...
Cross schemes for the P{w a } assay when testing the role of the DmBlm helicase in double-strand gap repair. (a) Females that are homozygous for P{w a } and heterozygous for one allele of mus309 are...
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Diagrammatic representation of ends-out targeting. Transgenic donor shows a...
Diagrammatic representation of ends-out targeting. Transgenic donor shows a clone using P[w35] as a basis for targeting. Hatched boxes are I-SceI recognition sites, half-arrows are FRTs, arrowheads...
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Vectors used for ends-out and ends-in targeting. Arrowheads are P-element...
Vectors used for ends-out and ends-in targeting. Arrowheads are P-element ends, half-arrows are FRT sequences, hatched boxes are I-SceI and I-CreI recognition sites, open boxes are sites for cloning...
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Diagrammatic representation of ends-in targeting. In the transgenic donor,...
Diagrammatic representation of ends-in targeting. In the transgenic donor, hatched boxes are I-SceI and I-CreI recognition sites, half-arrows are FRTs, arrowheads are P-element ends. Black box is the...
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Outcomes of the repair of a double-strand break (DSB) introduced by I-SceI....
Outcomes of the repair of a double-strand break (DSB) introduced by I-SceI. (A) The integrated pim1-Q vector. Open arrows indicate the positions of PCR primers that are used to distinguish between the...
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Schematic representations of the (A) pim1-Q and (B) Rb-O targeting vectors....
Schematic representations of the (A) pim1-Q and (B) Rb-O targeting vectors. (A) Gene targeting strategy at the pim1 locus. Expression of the promotorless hygromycin resistance relies on the endogenous...
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